Procedure

This procedure will create a digestion report and send the result to the Output window. A maximum of four restriction enzymes may be used to digest at the same time.

1. Proceed to the selection of restriction enzymes in the Restriction Enzyme Window if necessary.

If the Restriction Enzyme Window is not open, the set of prototypes (all enzymes without isoschizomers) will be used.

2. Choose Analysis > Restriction Analysis > Digestion Report in the main menu or Sequence Analysis > Restriction Analysis > Digestion Report in the selection contextual menu.

A sequence must be selected prior to operate a restriction analysis, e.g. click Ctrl-A to selected all the nucleotides in the sequence viewer first.

3. Alternatively click on the Digestion Report button () in the Restriction Enzyme Window operation toolbar. If the button is not active, reselect the sequence or activate the window containing the sequence, like for example a sequence node in the Explorer Window, before click in the toolbar.

4. Open the Output multitab window to access the result.

Example of output

The following report was generated by cutting the IgG4 Fc with EarI and SmaI:

      IgG4_FC  •  Digestion Report
      DNA      768 bp   atgaaaaagaca ... ccgggtaaatga
      Number of restriction enzymes tested: 2
      
      Digestion with: SmaI, EarI
      
      Fragments (bp)  Enzyme      Position    Enzyme      Position
      333             EarI             155    SmaI             488
      257             SmaI             488    EarI             745
      155             -                  1    EarI             155
      23              EarI             745    -                768
      
      All sites were found.